WebTBK1 phosphorylation is thought to be an autoactivation event. Biochemical analysis demonstrated that the kinase domain alone was sufficient to fully autoactivate TBK1 and … WebApr 7, 2024 · Thirty minutes after AO, TBK1 activation at Ser 172 also became apparent and evidence of Parkin substrate (CISD1) ubiquitylation and LC3 maturation was also observed ( Fig. 2B ). At 60 min after AO, PINK1-phosphorylation of ubiquitin Ser 65 was increasingly apparent and continued to increase at later time points.
Interruption of post-Golgi STING trafficking activates tonic …
WebPhospho-AMPKα (Thr172) (40H9) Rabbit mAb detects endogenous AMPKα1 only when phosphorylated at threonine 183 and endogenous AMPKα2 only when phosphorylated at threonine 172. The antibody does … WebNov 15, 2024 · Western blot analysis of biochemical features of the cGAS-STING pathway showed strong activation of p-Sting, p-Tbk1, p-Stat1 in Gcc2KO but not Gcc2WT, Gcc2KOcGasKO and Gcc2KOStingKO cells. ISG... d49 schoology sign in
AMPK/ULK1-mediated phosphorylation of Parkin ACT domain
WebConfocal immunofluorescent analysis of THP-1 cells, untransfected (left) or transfected with Poly (I:C) (2.5 μg/ml, 6 hr; right), using Phospho-IRF-3 (Ser386) (E7J8G) XP ® Rabbit mAb (green) and β-Actin (13E5) Rabbit mAb (Alexa Fluor ® 647 Conjugate) #8584 (red). Blue = Hoechst 33342 #4082 (fluorescent DNA dye). Show More WebFigure Legend Snippet: TAOK1 promotes TBK1-IRF3 complex formation. a HEK293T cells were transfected with plasmids encoding HA-TBK1, Flag-IRF3, and varying doses of a plasmid encoding Myc-TAOK1 (0.5, 1.0, and 1.5 μg). Cells were harvested 24 h after transfection for immunoblot analysis as indicated with the antibody to Flag tag. b … WebDec 9, 2013 · IPS-1 and STING localize to the mitochondria and endoplasmic reticulum, respectively, whereas it is unclear where phosphorylated TBK1 is localized in response to … d49m36sb specs